ABSTRACT
<p><b>OBJECTIVE</b>To investigate whether the ERK/FoxO3a signal axis could induce the inhibitory effect of vitexin 1 (VB-1) in HepG2 cell proliferation.</p><p><b>METHOD</b>The MTT method was adopted to observe the effect of different concentrations of VB-1 on human hepatoma carcinoma cell line HepG2 and immortalized human embryo liver cell line L-02. The cell growth was assessed by the clone formation assay. The protein phosphorylation levels of ERK1/2 and FoxO3a were measured by the western blot.</p><p><b>RESULT</b>VB-1 inhibited the viability of HepG2 cell line in a concentration-dependent manner, with a weak effect on L-02 cell line. VB-1 could effectively inhibit the anchorage-dependent growth of HepG2 cells, and reduce the expression levels of pERK1/2 and pFoxO3a in a concentration-dependent manner. MEK1/2 inhibitor PD98059 could enhance VB-1' s effect in inhibiting HepG2 cell proliferation and ERK1/2, FoxO3a phosphorylation.</p><p><b>CONCLUSION</b>VB-1 inhibits the proliferative activity of hepatoma carcinoma cell line HepG2 by blocking the ERK/FoxO3a signal axis.</p>
Subject(s)
Humans , Apigenin , Pharmacology , Carcinoma, Hepatocellular , Drug Therapy , Genetics , Metabolism , Cell Proliferation , Drugs, Chinese Herbal , Pharmacology , Extracellular Signal-Regulated MAP Kinases , Genetics , Metabolism , Forkhead Box Protein O3 , Forkhead Transcription Factors , Genetics , Metabolism , Growth Inhibitors , Pharmacology , Hep G2 Cells , Liver Neoplasms , Drug Therapy , Genetics , Metabolism , Signal TransductionABSTRACT
<p><b>OBJECTIVE</b>To study the permeability of intact mouse abdominal skin to aniline and the protective capability of two typical lab gloves against aniline.</p><p><b>METHODS</b>A Franz diffusion cell was used to perform in vitro transdermal absorption test and glove permeation test for aniline (0.102 mg/ml and 0.010 mg/ml). The permeabilities of intact mouse abdominal skin and gloves to aniline were measured by high performance liquid chromatography-diode array detection.</p><p><b>RESULTS</b>The transdermal penetration of the two concentrations of aniline followed zero order kinetics within 12 h, exhibiting total aniline permeabilities within 24 h of 51.71% and 48.31%, respectively. The absorption liquid had an aniline concentration of at least 18 µg/L. The medical disposable latex glove could not stop the penetration of 0.010 mg/ml aniline, but the industrial natural latex glove could.</p><p><b>CONCLUSION</b>The penetration of 0.102 mg/ml and 0.010 mg/ml aniline through the mouse abdominal skin follows zero order kinetics within 12 h. The medical disposable latex glove cannot stop the penetration of 0.010 mg/ml aniline, but the industrial natural latex glove can.</p>
Subject(s)
Animals , Mice , Aniline Compounds , Pharmacokinetics , Toxicity , Gloves, Protective , Skin AbsorptionABSTRACT
<p><b>OBJECTIVE</b>To in vestigate the chemical constituents of Sarcandra glabra and obtain a more comprehensive understanding on its effective components.</p><p><b>METHOD</b>The constituents were isolated by various column chromatographic method and their structures were elucidated by physico-chemical properties and spectroscopic analysis.</p><p><b>RESULT</b>Five flavonoid glycosides were isolated and identified as kaempferol-3-O-beta-D-glucuronide (1), quercetin-3-O-alpha-D-glucuronide (2), quercetin-3-O-beta-D-glucuronopyranoside methyl ester (3), 5, 7, 4'-trihydroxy-8-C-beta-D-glucopyranosyl flavanone (4), neoastilbin (5), 5-O-caffeoylquinic acid methyl ester (6), 3, 4-dihydroxybenzoic acid (7), isofraxidin (8).</p><p><b>CONCLUSION</b>Compounds 1-6 were isolated from the genus Sarcandra for the first time. The glucuroide compounds compounds 1-3, were first isolated from the genus Sarcandra.</p>
Subject(s)
Caffeic Acids , Chemistry , Coumarins , Chemistry , Drugs, Chinese Herbal , Chemistry , Flavonoids , Chemistry , Glucuronides , Chemistry , Glycosides , Chemistry , Magnetic Resonance Spectroscopy , Magnoliopsida , Chemistry , Spectrometry, Mass, Electrospray IonizationABSTRACT
<p><b>OBJECTIVE</b>To investigate the chemical constituents of Oldenlandia diffusa.</p><p><b>METHOD</b>The column chromatography with polyamide Sephadex LH -20, silica gel as packing materials and HPLC, were used to separate and purify the chemical components. The structures were elucidated on the basis of physicochemical properties and spectral data.</p><p><b>RESULT</b>Nine compounds were isolated and identified as 2, 6-dihydroxy-1-methoxy-3-methylanthraquinone (1), 2-hydroxy-1-methoxy-3-methylanthraquinone (2), 2-hydroxy-3-methylanthraquinone (3), quercetin-3-O-[2-O-(6-O-E-sinapoyl)-beta-D-glucopyranosyl]-beta-glucopyranoside (4), quercetin-3-O-[2-O-(6-O-E-feruloyl)-beta-D-glucopyranosyl]-beta-glucopyranoside (5), kaempferol-3-O-[2-O-(6-O-E-feruloyl)-beta-D-glucopyranosyl]-beta-galactopyranoside (6), quercetin-3-O-(2-O-beta-D-glucop-yranosyl)-beta-D-glucopyranoside (7), rutin (8) and quercertin (9).</p><p><b>CONCLUSION</b>Compounds 1 and 8 were obtained from this plant for the first time, and compound 1 was a new compound.</p>
Subject(s)
Anthraquinones , Chemistry , Molecular Conformation , Molecular Structure , Oldenlandia , Chemistry , Plants, Medicinal , Chemistry , Quercetin , Chemistry , Rutin , ChemistryABSTRACT
<p><b>AIM</b>To study the chemical constituents of Polygala aureocauda Dunn..</p><p><b>METHODS</b>Chemical compounds were isolated by column chromatography and their structures were determined mainly by spectroscopic means (UV, IR, MS, 1HNMR, 13CNMR, HMQC, HMBC).</p><p><b>RESULTS</b>Three compounds were isolated and identified as 3-hydroxy-1,4-dimethoxyxanthone (I), 1, 7-dihydroxy-2, 3-methylendioxyxanthone (II), 7-hydroxy-1-methoxy-2, 3-methylendioxyxanthone (III).</p><p><b>CONCLUSION</b>Compounds I-III were isolated from Polygala aureocauda Dunn. for the first time, whereas compound I is a new xanthone.</p>